The Eastern Strawberry tree, Arbutus andrachne L., is a medicinal evergreen small tree naturally distributed from Eastern Mediterranean to the Northern Black Sea region. In Palestine, the tree is known for its high medicinal value and recently has been included within the endangered species. For conservation and utilization of A. andrachne we investigated the presence of catechin, an antioxidant and active flavonoid in the ethylacetate fraction in leaves of wild plant material and also in the extract of callus and the in vitro grown vegetative tissues. HPLC analysis of catechin revealed 0.063% in callus extract, 2.5% in the in vitro growing tissues and 0.5% in wild growing plants. In vitro propagation and callus culture are promising approaches for the secondary metabolites production in the case of A. andrachne.